Volume 10, No 1, Mar 2000

ISSN: 1001-0602 
EISSN: 1748-7838 2018 
impact factor 17.848* 
(Clarivate Analytics, 2019)

Volume 10 Issue 1, March 2000: 71-80

ORIGINAL ARTICLES

Using a non-radioisotopic, quantitative TRAP-based me- thod detecting telomerase activities in human hepatoma cells

ZHANG Ru Gang, Xing Wang WANG, Jin Hui YUAN, Li Xia GUO, Hong XIE*

Shanghai Institute of Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China Correspondence:

A non-radioisotopic, quantitative TRAP-based telomerase activity assay was established mainly by using SYBR Green-I staining instead of radioisotope. Comparing with conventional radioisotope based method, it was better in reproducibility and accuracy. Using this method, we found telomerase activities were absent in normal human liver cells, while detected in all of four human hepatoma cell lines (BEL-7404, SMMC-7721, QGY-7903 and HCCM) without significant differences.

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