Volume 9 Issue 4, December 1999: 255-260
ORIGINAL ARTICLES
The structure of the nucleosome core particle of chromatin in chicken erythrocytes visualized by using atomic force microscopy
ZHAO Hui1, Yi ZHANG2, Shu Bing ZHANG1, Chu JIANG1, Qi Ye HE1, Min Qian LI2, Ruo Lan QIAN1,*
1. Shanghai Institute of Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China
2. Shanghai Institute of Nuclear Research, Chinese Academy of Sciences, Shanghai 201800, China
Correspondence:
The structure of the nuclosome core particle of chromatin in chicken erythrocytes has been examined by using AFM. The 146 bp of DNA wrapped twice around the core histone octamer are clearly visualized. Both the ends of entry/exit of linker DNA are also demonstrated. The dimension of the nucleosome core particles is ~ 1-4 nm in height and ~ 13-22 nm in width. In addition, superbeads (width of ~ 48-57 nm, height of ~ 2-3 nm) are occasionally revealed, two turns of DNA around the core particles are also detected.
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