Volume 9 Issue 1, March 1999: 61-70
ORIGINAL ARTICLES
Tropomyosin is localized in the nuclear matrix and chromosome scaffold of physarum polycephalum
ZENG Xian Lu1, Ming Da JIAO1, Miao XING1*, Xiao Guang WANG2, Shui HAO1
1Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024, China
2Department of Biology, Changchun Normal College, Changchun 130032, China
Correspondence:
The nuclei and chromosomes were isolated from plasmodia of Physarum polycephalum. The nuclear matrix and chromosome scaffold were obtained after the DNA and most of the proteins were extracted with DNase I and 2 M NaCl. SDS-PAGE analyses revealed that the nuclear matrix and chromosome scaffold contained a 37 kD polypeptide which is equivalent to tropomyosin in molecular weight. Immunofluorescence observations upon slide preparations labeled with anti-tropomyosin antibody showed that the nuclear matrix and chromosome scaffold emanated bright fluorescence, suggesting the presence of the antigen in them. Immunodotting results confirmed the presence of tropomyosin in the nuclear matrix and chromosome scaffold. Immunoelectron microscopic observations further demonstrated that tropomyosin was dispersively distributed in the interphase nuclei and metaphase chromosomes.
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