Volume 8 Issue 3, September 1998: 195-207
ORIGINAL ARTICLES
Phosphorylation and biosynthesis of high molecular weight proteins of tumor nuclear matrix.
Bazarnova TM, Buldyaeva TV, Filatova LS, Akopov SB, Zbarsky IB.
NK Koltzov Institute of Developmental Biology, Russian Academy of Sciences, Moscow, Russia.
Correspondence: Zbarsky IB.
Our previous studies showed a predominance of high molecular weight protein group in tumor nuclear matrices. Contrary to normal cells, proteins of this group are preferentially phosphorylated. Phosphoproteins of hepatoma nuclear matrix are selectively subjected to rapid proteolysis. By alkali treatment and a monoclonal antibody against phosphotyrosyl residue the presence of two high molecular weight bands of phosphotyrosyl-containing proteins was detected in nuclear matrices of tumor but not of normal liver cells. High molecular weight protein group of tumor nuclear matrices revealed also a rapid turnover and preferential incorporation of labeled amino acids selectively inhibited by chloramphenicol.
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