Volume 6 Issue 2, December 1996: 115-124
ORIGINAL ARTICLES
Low temperature induces oocytes P34cdc2 synthesis and accumulation - the acquisition of competence to resume meiosis in toad oocytes
LU JI NING,ZHENG GU,HONG SHENG,CHUANG JU LIU,JIA KE TSO
Shanghai Institute of Cell Biology, Chinese Academy of Sciences, 320 yueyang Road, Shanghai 200031, China
Correspondence: JIA KE TSO
Full grown oocytes derived from Bufo Bufo gargarizans rearing at high temperture environment (24’C). never underwent GVBD after progesterone treatment . No P34cdc2 H1 kinase activity was detected in the oocytes after progesterone stimulation or OA microinjection;Western blotting analysis showed that the level of P34cdc2 and P33 in the oocytes are significantly lower than those in the oocytes derived from the hibernating toads (between 10 ‘C). 35 S-Met incorporation analysis showed that when the oocytes derived from the hibernating toads (below 10’C). 35S-Met incorportation analysis showed that when the oocytes were incubated at 6’C, synthesis of about thirty defferent polypetides was promoted or induced , including P34cdc2 and some other P13suc1-binding proteins. All these results indicated that a low temperature environment is essential for the oocytes of Bufo Bufo gargarizans to express and store some cell cycle drivers and its regulators , and to gain the maturation competence. These results will also provide a new clue for explaining the molecular mechanisms why gametogenesis of some organisms depends on a relative low temperature how to maintain the geographical distributin of some animals.
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