Volume 6 Issue 2, December 1996: 137-144
ORIGINAL ARTICLES
Induction of apoptosis and change of bel-2 expression in macrophage Ana-1 cells by all-trans retinoic aicd
Induction of apoptosis and change of bel-2 expression in macrophage Ana-1 cells by all-trans retinoic aicd
Shanghhai Institute of Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China
Correspondence: GANG PEI
Macrophage cells play an important role in the initiation and regulation of the immune response . All-transretinoic acid (ATRA) and its natural and synthetic analogs (retinoids) affect a large number of biologyal processes. Recently,retinoids have been shown promise in the therapy and prevention of various cancers. However,many interesting questions related to the activities of retinoids remain to be answered: (I) Molecular mechanisms by which retinoids exert their effects; (II) shy the clinical uses of varying severity with a higher frequency of blood system symptoms ;(III) little is known for its impacts on macrophage cells etc. we set up this experiment ,therefore, to examine the apoptosis of ATRA on macrophage Ana-1 cell line .Apoptosis of the cells was quantitated after staining cells with propidium iodide (PI) ,by both accounting nuclear condensation and flow cytometry. When the cells were treated with ATRA at or higher htan 1 µM for more than 24h ,sighificant amount of the apoptotic cells was observed . Induction of apotosis of Ana-1 cells by ATRA was in time –and dose –dependent manners,exhibiting the similar pattern as the apoptosis induced by actinomycin D (ACTD) . ATRA treatment of Ana-a cells also caused the changes of the mRNA levels of ATRA- induced apoptosis and change of bcl-2 in Ana-1 cells
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