Volume 6 Issue 2, December 1996: 155-166
ORIGINAL ARTICLES
Resersion of malignancy in human gastric cancer MKN - 45 cells through the transfection of transforming growth factor-βtype II receptor gene
SUN HONG,WEI KANG SHI, ZHEN YAO
Shanghhai Institute of Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China
Correspondence: SUN HONG
Human gastric cancer MKN-45 cells which are resisrant to TGR-ßtype I and type III receptors ,but not type II receptors ,have been used as model system to reconstitute these cancer cells with TGR-ßRII cDNA . The results of these experiments indicated that the reexpression of TGF-ßRII gene in MKN –45 cells can restore their sensitivity to TGF-ß growth inhibition ,decrease their growth rate, reduce their cloning efficiency in soft agar and tumorigenicity in nude mice in stable transfectants, in comparison with their control MKN-45 cells ,Among different RII transfectants, dheir defference in the changes of these parameters , as a result of the regain of autocrine negative growth control by TGF-ß, is roughly proportional to their level of expression of transfected RII mRNA . From these data , it is concleded that the inactivation of TGF-ß RII gene is related to the excape of growth control by TGF-ß in MKN-45 cells . The importance of the study of the interplay of TGF-ß and tis receptor system in the nagative growth control of gastric cancer , and possibly also of other cancers , is discussed.
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