Volume 6 Issue 2, December 1996: 177-187
ORIGINAL ARTICLES
Selective proliferation of human ?d T cells in vitro
CHEN SONG HUA, AKINORI OKI
TADAO OHNO,SATOKO ISHIKAWA,MASATAKA MOCHIZHKI,YU FANG CHE,HUI MING DAI , XI RUI GE
Shanghai Institute of Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China
Richen Cell Bank, Koyadai,Tsukuba Sciences City, Japan
Department of Gynecology, Tsukuba University, Amakubo ,Tsukuba Science City, Japan
Kyoritsu College of Pharmacy ,Shibakoen, Minato-ku , Tokyo, Japan
Correspondence: CHEN SONG HUA
The effect of monoethylphosphate (MEP, commercial available or synthesized ) together with IL –2 on the selective proliferation of human?d T cells in vitro from peripheral blood mononuclear cells (PBMC) of healthy donors and of cancer patients was investigated . The?d T cells were stimulated by MEP to proliferate in a dose –dependent manner . The effect of synthesized MEP was 10 times greater than that of commercial MEP . When the total cell number increased about 1000-3000 fold ; and the ration of?d T cells reached to 70%-80%. The selective expansion of?d T cells depended on the synergic action of MEP and IL-2. The bulk cultured?d T cells cells exhibited obvious cytotoxic activities against allogenic tumor cells .The culture system described here not only offers a simple method for obtaining a large number of?d T cells which may become a new effector in the adoptive immunotherapy, but also provides a useful model for the further studies of the structure and function of?d T cells in vitro.
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