Volume 6 Issue 2, December 1996: 189-199
ORIGINAL ARTICLES
Abrupt decrease of c-myc expression by antisense transcripts induced terminal differerntiation and apoptosis in human promyelocytic leukemuia HL-60 cells
HAO XIU JUAN,PEI HSIEN TANG, XIU SEN LI FEI ZI JIANG, YONG ZHI XI, NING MAO ,DE LIU DU , MIN WU
Institute of Basis Medical Sciences ,Beijing 100850
National Laboratory of Molecular Oncology, Institute of Cancer Research Beijing, Chinese Academy of Medical Sciences, Beijing 100021
Correspondence: PEI HSIEN TANG
This study was designed using c-myc antisense transcripts to evaluate how alteration of c-myc expression in human myeloid leukemic HL-60 cells could influence the myelomonocytic differentiation and induction of apoptosis. The recombinant plasmid pDACx expressing antisense transcripts to c-myc fragment containing a part of inron 1 and 137 nt exon 2 was constructed. PDACx was transfected into HL –60 cell line by lipofectin , peroxidase and a-NAE as well as detection of CD13 and CD33 antigens by flow cytometric analysis indicated occurrence of myelomonocytic differentiation in cells expression antisense transcripts to c-myc. DNA degradation measured by DNA gel electrophoresis and typical morphological changes observed under electron microscope proved the switch –on of apoptosis in terminally differentatin HL –60 cells.
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