Volume 6 Issue 1, June 1996: 1-9
ORIGINAL ARTICLES
Construction of normal human IgE phage antibody library and the screedning of the anti-trichosanthin IgE
ZAN HONG, MING YEH
Shanghai Institute of Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China
Correspondence:
Allergen specific IgE response is the major cause of immediate hypersensitively. However the number of IgE-priducing B cells and the amount of IgE , especially the soecific IgE ,are not low, it greatly impedes the study of the allergic-specific antibody responses. Here we report the construction of a normal huamn IgE combinatorical libarary . The repetorie of IgE VH genes and of k gene were separately amplified from normal human peripheral blood lymphocytes fhrough RT-PCR,and were then constructed to form the phage surface display humanFab(IgEVH) library. A plant protein allergen, trichosanthin(TCS), was used to affinity-enrich and to secrren the anti-TCS phage HuFab clones from the library. Human IgE(Fab) to TCS were detected.
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