Volume 6 Issue 1, June 1996: 23-30
ORIGINAL ARTICLES
Correlation between inhibition of calcium -dependent apoptosis by cyclosporin A and calcium transportation in HL-60 cells
HUANG QI QING, MING FANG, HONG QING ZHANG, SHAO BAI XUE
Department of Biology, Beijing Normal University, Beijing 100875 , China.
Correspondence: SHAO BAI XUE
Both calcium ionophore A23187 and endoplasmic reticulum Ca2+- ATPase inhibitionr thapsigargin (TG) could increase intracellular free calcium concentration and induce apoptosis in some cell lines . In the present study, we found thar HL-60 cells treated with A23187 (1µg/ml) for 4 h or with TG (0.5 ug/ml) for 2 h showed typical characteristics of apoptosis. Pretreatment with nontoxic concentration of cyclosporin A(CsA) (1ug/ml) could block these effects. Flow cytometric analysis of intracellular Ca2+ after staining with fluo-3 AM showed that CsA did not prevent the increase of intracellular calcium induced by A23187 or Tg, but it could maintain the high level of intancellular Ca2+ for a long time . These results suggest that CsA may prevent calcium- induced apoptosis by blocking the transportation of Ca2+ in HL-60cells.
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