Volume 6 Issue 1, June 1996: 47-53
ORIGINAL ARTICLES
Effect of captopril or verapamil on intracellular sodium in cultured vascular smooth muscle cells.
QI JIAN HUA, LU ZHUANG, JUN WANG, MIN LU, XIN MING WANG, ZHENG JUN JIN
Shanghai Institute of Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China
Department of Parmacology , Shanghai Second Medical University, Shanghai 200025, China.
Correspondence:
The effects of captopril (Cap) and verapamil (Ver) alone and in combination on intracellular Na+ concentration([Na+]i) in cultured aortic smooth muscle cells (ASMC) of rabbits was evaluated by a direct measurement of [Na+] I with fluorescent dye sodium-binding benzofuran isophthalate (SBFI) combined with digital. [Na+]I in resulting cells was found to be 11.9±0.7 mmol/L. Angiotensin II (Ang-II, 0.1-10 umol/L) induced an increase of [Na+]I in concentration-dependent manner. Ver(0.1-10 µmol/L) inhibited Ang-II (1µmol/L)-induced increase in [Na+]I while Cap enhanced Ang-II-induced increase in [Na+]I at 10µmol/L but not at 0.1-1µmol/L. Ver(0.1-1µmol/L) abolished enhancement of Ang-II-induced increase in [Na+]I by Cap . Thus , the inhibition of Cap-enhanced[Na+]I byVer may provide a new hypothesis for the underlying molecular mechanism of synergistic effect of the combination of Ca2+ antagonists and angiotensin coverting enzyme inhibitors in controlling blood pressure.
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