Volume 6 Issue 1, June 1996: 55-63
ORIGINAL ARTICLES
Can iverexpression of TCG-β1 gene change the sex ratio in transgenic mice
TSUNG HSIAO CHEN, CHIEN,JIE XU,LU XIA XU,XIU LAN LI,WEI KANG SHI, ZHEN YAO
Shanghai Institute of Cell Biology, Chinese Academy of Sciences , Shanghai 200031,China
Correspondence:
Mouse TGF-ß1 gene was microinjected into male pronuclei of F2 Hybrid fertilized eggs obtained by mating CSJLF1 and C57BL/6J inbred strains to generate transgenic mice with over-expressed TGF--ß1 gene. The rate of found production is 31% and Southern blot analysis of founder mice tail DNAs gave an integrated to the chromosomes of transgenix mice and transmitted to their progeny at a rate of 33% in the second generation. Dot blot analysis of tail RNA of some transgenic mice indicated a moderate expression of the transgene. The most interesting finding of the present work is the striking deviation from the normal male: female sex ration in transgenic mice, with an average ration of 6.7:1. The possible nature of the predominance of male sex in transgenic mice overexpressing TGF-ß1 is discussed.
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