Volume 6 Issue 1, June 1996: 66-73
ORIGINAL ARTICLES
Molecular cloning and primary sequence analysis of a gene encoding a putative chitinase gene in Brassica oleracea var . capitata
TANG GUO QING, YONG YAN BAI,SHI WEI LOO
Shanghai Institute of Plant Physiology, Chinese Academy of Sciences , 300 Frenglin Road Shanghai, China 200032
Correspondence:
Chitinase which catalyzes the hydrolysis of the ß-1, 4-acetyl-D-glucosamine linkages of the fungal cell wall polymer chitin , is involved in inducible plants defense system. By construction of cabbage (Brassica oleracea var. capitata) genomic library and screening the library with pRCH8,a probe of rice chitinase gene fragment, a chitinase genomic sequence was isolated . the complete nucleotide sequence of the putative cabbage chittnase gene (cabch29) was determined, with its longest open reading frame (ORF) encoding a polypeptide of 413 aa . This polypeptide consists of a 21 aa N-terminal signal peptide, two chitin-binding domains different from those of other classes of plant chitinases , anda catalytic domain. Homology analysis illustrated that this cabch29 gene has 58.8% identity at the nucleotide level with the pRCH8 ORF probe and has 50% identity at the amino acid level with the catalytic domain of chitinase from bean, maize and sugar beet. Meanwhile several kinds of cis-elements, such as TATA box,CAAT box, GATA motif, ASF-1 binding site, would-response elements and AATAAA,have also been discovered in the flanking region of cabch29 gene.
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