Volume 6 Issue 1, June 1996: 75-84
ORIGINAL ARTICLES
Functional properties of a chitinase promoter from cabbage (Brassica oleracea var. capital)
TANG GUO QING, YONG YAN BAI,SHI WEI LOO
Shanghai Institute of Plant Physiology, Chinese Academy of Sciences , 300 Frenglin Road Shanghai, China 200032.
Correspondence:
The 5’-region of the chitinase gene cabch29,derived from Brassica oleracea var. capitata, has been sequenced and analyzed for cis-acting elements important in controlling gene expression in transgenic tobacco plants . Different 5’-deletion fragments were linked to reporter gene ß-glucuronidase (GUS) as translational fusions, and the expression of the expression of these chimeric genes was analyzed in vegetative organs and tissues. Sequences up to-651 showed some basal GUS activity woth nearly equal levels in wounded and intact tissues. The addition of further upstream sequences (-651 to –1284) enhanced expression level , and the expression driven by this fragment was inducible by a factor of two to three-fold by wounding . Histochemical analysis of different tissue from transgenic plants that contain cabch29 promoter-GUS fusion gene demonstrated wound-inducible and tissue-specific cabch29 promoter activity in plants containing the 1308 base pair fragment. The location of GUS activity appears to be cells of stem, leaf and roots. Meanwhile , the temporal and spatial expression of cabch29-GUS fusion gene has been investigated . Among the different vegetative organs, a high level of GUS activity was observed in stem and a moderate one on roots; whereas , wounding stress led to a high level of GUS in wtem and moderate one in leaf.
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