Volume 5 Issue 2, December 1995: 165-179
ORIGINAL ARTICLES
Visualization of Golgia apparatus as an intracellular calcium store by laser scanning confocal microscope.
CUI JIE, YAN LI, SHAOBAI XUE
Biology Department, Beijing Normal University, Beijing 100875, China
Correspondence: SHAOBAI XUE
Using laser scanning confocal microscopy, we have found that the in cells loaded with fluo-3/AM, highest instracellular Ca2+ in living human fibroblasts exposing to calcium-free medium in response to agonists has been investigated. PDGF, which releases Ca2+ from intracellular stores by inositol(1, 4, 5)-triphosphate pathway, produced a biphasic transient rise in intracellular calcium. The initial rise was resulted from a direct release of calcium from the Golgi apparatus. Calcium could be also released from and reaccumulated into the Golgi apparatus by the stimulation of thapsigargin, an inhibitor of the Ca2+ transport ATPase of intracellular calcium store. Permeablizing the plasma membrane by 10 uM digitonin resulted in the calcium release from the Golgi apparatus and depletion of the internal calcium store. These results suggest that the Golgi apparatus plays a role in Ca2+ regulation in signal transduction.
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