Volume 5 Issue 2, December 1995: 181-186
ORIGINAL ARTICLES
Apoptosis of human leukemic HL-60 cells induced to differentiate by treatment with RA or DMSO
Apoptosis of human leukemic HL-60 cells induced to differentiate by treatment with RA or DMSO
Key Laboratory of Cell Proliferation and Regulation Biology, Beijing Norman University, Beijing 100875, China.
Correspondence:
In present study, we studied the effect of all-trans retinoic acid(ATRA) and dimethylsulfoxide (DMSO) on the induction of apoptosis in HL-60 cell line. Based on morphological changes by Hochest 33342 staining and identification of internucleosomal DNA cleavage by gelelectrophoresis, we observed aberrant nuclear chromatin condensation and ladder-like pattern of DNA degradation. Using Flow Cytometric method, we found sub-G1 peak in RA-treated HL-60 cells starting 5 to 6d after the initiation of the treatment. However, such an obvious apoptotic peak was not identified in DMSO-differentiated cells. Combining the research accomplished before, our study approves further that apoptosis could be a common mode of death of terminally differentiated HL-60 cells.
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