Volume 5 Issue 2, December 1995: 187-195
ORIGINAL ARTICLES
Plant regeneration from protoplasts of hydroxyproline resistant cell line in Onobrychis viciaefolia
XU ZIQIN, JINGFEN JIA
Cell Biology Laboratory, Lanzhou University, Lanzhou 730000, China.
Correspondence: JINGFEN JIA
An efficient protocol for plant regeneration from protoplasts of hydroxyproline(HYP) resistant cell line of Onobrychis viciaefolia was established. In SH medium supplemented with 1 mg/L 2,4-dichlorophenoxy-acetic acid (2,4-D), 0.5 mg/L kinetin (KT) and 0.2 mg/L naphthalene acetic acid (NAA), the division frequency of protoplast-derived cells reached up to over 60%, and microcalli were obtained in 5-6 wk. Upon transferring them on agar solidified MS medium plus 2 mg/L indole-3-acetic acid (IAA), shoots were induced. After cultivating them on MS medium with or without IAA, roots were regenerated. Chromosome number of ass protoplast-regenerated plants examined were Norman (2n=28). The protoplast-derived calli and plants grew vigorously on the medium containing 10 mmol/L HYO.
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