Volume 5, No 1, Jun 1995

ISSN: 1001-0602 
EISSN: 1748-7838 2018 
impact factor 17.848* 
(Clarivate Analytics, 2019)

Volume 5 Issue 1, June 1995: 1-7

ORIGINAL ARTICLES

Chromosomal localization of ribosomal DNA sequences in an apple rootstock using a digoxygenin detection system

ZHU JIMEI , S E GARDINER

The Horticulture and Food Research Institute of New Zealand Limited
Mt Albert Research Centre,Private Bag 92 196, Auckland, Nes Zealand
Batchelar Research Centre, Private Bag 11 030, Palmerston North, Nes Zealand Correspondence:

A 6kb rDNA probe comprising the 18s coding plus spacer sequences has been hybridized to the metaphase chromosomes of apple rootstock cultivar MM106 demonstrating the localization of ribosomal gene arrays in the vicinity of the telomeric regions of the short arms of chromosomes 6 and 14. The in situ results using digoxygenin labeling coupled to an alkaline phosphatase immunoassay were confirmed by silver staining for NORs and nucleoli. This study demonstrates the feasibility of molecular cytogenetic analysis of very small chromosomes (1.0-2.7 um) of apple.

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