Volume 5 Issue 1, June 1995: 35-45
ORIGINAL ARTICLES
TGF-B receptors in mouse ES-5 cells and their differentiated derivatives
SHI WEIKANG, JUN WU, LUXIA XU, HSIAOCHIEN TSUNG..
Shanghai Institute of Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China
Correspondence:
By radioreceptor binding studies with iodinated BGF-B1, it has been shown that an undifferentiated ES-5 cell expresses approximately 3270 receptors with a dissociation constant Kd=130pM, but after the induction of differentiation by retinoic acid and dBcAMP, the receptor number of a differentiated RA-ES-5 cell was increased about 80% and the Kd was also increased to 370pM. Furthermore, more direct evidence supporting the expression of TGF-B type I and type II receptors in both ES-5 and RA-ES-5 cells has come from dot blot hybridization of cellular mRNA with cDNA probes for type I and type II receptors. Meanwhile, mRNA expression level of types I and II receptors in RA-ES-5cells were higher than that in ES-5 cells. Down-regulation of TGF-B receptors with a significant decrease in the rate of cell proliferation in both cells, was found by employing a pretreatment with neutralizing antibody to TGF-B1. The possible role of receptors for TGF-B in cell differentiation is discussed here.
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