Volume 5 Issue 1, June 1995: 67-74
ORIGINAL ARTICLES
The influence of Trichosanthin on the induction of IgE responses to ovalbumin under adjuvant-free condition.
JI YONGYONG, CUIHONG YANG, MING YEH
Shanghai Institute of Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China.
Correspondence:
Trichosanthin(TCS) is a potent allergen in mice. It can reproducibly induce specific IgE responses in C57BL/6J mice without the help of adjuvant alum. TCS can bring out the IgE responses to ovalbumin(OVA), while OVA it self could not induce IgE responses to it. However, TCS only works when OVA immunization is given one day after TCS immunization. Either time lag in OVA immunization, or immunization of both antigens at the same time, or OvA immunization given first, all has no effect on the induction of IgE responses to OVA. Through analysis of the antibody specificity of hybridoma clones, it indicated that specific antibodies to TCS of OVA were secreted by independent B cell clones. The IgE antibodies showed no polyreactivity to different antigens.
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