Volume 5 Issue 1, June 1995: 75-91
ORIGINAL ARTICLES
FT I, a novel positive myeloid-lineage-specific transcription regulatory element within the mouse myeloperoxidase gene enhancer, En 1
AHU JINGDE
CRC Department of Medical Oncology, CRC Beatson Laboratories, Glasgow University, Garscube Estate, Switchback Road, Glasgow G61 1BD, United Kingdom.
Correspondence: AHU JINGDE(gpma66@udcf.gla.ac.uk )
FT I (AAAAGGGGAAGCAGAG), A POLY PURINE ELEMENT WITHIN THE MYELOID-LINEAGE SPECIFIC ENHANCER (En 1) of the mouse myeloperoxidase gene [1,2] has been further characterized. 1, FT I functions as a myeloid-lineage specific transcription regulatory element; 2, WEHI 3BD+cells have higher binding activity to FT I and express the proteins which could form the unique DNA-protein complex(es) of FR I; 3, The essential sequence for the specific DNA-protein interactions of FT I is AAAAGGGGAAGC;4, South-western analysis in conjunction with the competition assay of the proteins binding to FT I, has revealed a 28 kd protein in WEHI 3 BD= cells that displays the properties of the putative transcription factor which acts through FT I. These new findings have demonstrated both the functional myeloid-lineage specificity and the novelty of FT I.
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