Volume 4 Issue 2, December 1994: 145-153
ORIGINAL ARTICLES
EGF receptor-mediated intracellular calcium increasein human hepatoma BEL-7404 cells
Fu Tao, YongHua Xu, WanLI Jiang, HongYu Zhang, PeiHong Zhu, Jun Wu
Laboratory of Cellular and Molecular Oncology, Shanghai Institute of Cell Biology, Shanghai Institute of Physiology, Chinese Academy of Science, Shanghai 200031, China
Correspondence:
Epidermal growth factor (EGF) induced intracellular free calcium ([Ca~(2+)]i) response was studied in fura-2- or fluo-3-loaded human hepatoma cells of BEL-7404 cell line. Single cell [Ca2+]i analysis and [Ca2+], measurement in cell populations revealed that EGF triggered a rapid [Ca2+]iincrease in the dose-dependent and time- dependent manner. Pretreatment of cells with an endoplasmic reticulum (ER) Ca2+-ATPase inhibitor, thapsigargin (TG) at 100 nM concentration for 20 min, completely abolished EGF-induced [Ca2+]i increase, and chelating extracellular calcium by excess EGTA partially inhibited the increase.Furthermore, the expression of antisense EGF receptor sequence in BEL-7404 cells suppressed the [Ca2+]i response to EGF. The results suggest that EGF receptor-mediated [Ca2+]i increase in the human hepatoma cells is essentially dependent on the Ca2+ storage in ER.
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