Volume 4 Issue 2, December 1994: 163-172
ORIGINAL ARTICLES
Observation of nuclei reassembled from demembranatedXenopus sperm nuclei and analysis of their laminacomponents
Qu Jian, ChuanMao Zhang, ZhongHe Zhai.
College of Life Sciences, Beijing University, Beijing 100871, China.
Correspondence:
A cell-free preparation obtained from extracts of activated Xenopus laevis eggs induced chromatin decondensation and nuclear formation from demembranated Xenopus sperm nuclei. Electron microscopy revealed that the reassembled nucleus had a double-layered nuclear membrane, nuclear pore complexes, and decondensed chromatin etc. Indirect immunofluorescence analysis demonstrated the presence of lamina in newly assembled nuclei. Western-blotting results showed that lamin LII was present in egg extracts and in lamina of the reassembled nuclei which were previously reported to contain only eggderived lamin LIII.
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