Volume 4 Issue 1, June 1994: 97-108
ORIGINAL ARTICLES
High concentration of calcium ions in Golgi apparatus
Xue ShaoBai, M.Robert Nicoud, Jie Cui, D.J. Arndt Jovin.
Department of Biology, Beijing Normal University, Beijing 100875, China.
Max-Planck-Institute fur Biophysikalische Chemie, Gottingen, Germany
Correspondence: Xue ShaoBai
The interphase NIH3T3 cells were vitally fluorescent stained with calcium indicator fluo-3 and Glogi probe C6NBD-ceramide, and then the single cells were examined by laser scanning confocal microscopy (LSCFM) for subcellular distributions of Ca2+ and the location of Golgi apparatus. In these cells, the intracellular Ca2+ were found to be highly concentrated in the Golgi apparatus. The changes of distribution of cytosolic high Ca2+ region and the Golgi apparatus coincided with the cell cycle phase.In calcium free medium, when the plasma membrane of the cells which had been loaded with fluo-3/AM were permeated by digitonin, the fluorescence of the Golgi region decreased far less than that of the cytosol. Our results indicated that the Glogi lumen retained significantly high concentration of free calcium.
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