Volume 23, No 5, May 2013

ISSN: 1001-0602 
EISSN: 1748-7838 2018 
impact factor 17.848* 
(Clarivate Analytics, 2019)

Volume 23 Issue 5, May 2013: 645-657

ORIGINAL ARTICLES

A role for the RNA-binding protein MOS2 in microRNA maturation in Arabidopsis

Xueying Wu^1,2, Yupeng Shi^2,5, Jingrui Li^2,6, Le Xu⁴, Yuda Fang⁷, Xin Li⁸ and Yijun Qi^3,4

¹College of Life Sciences, Beijing Normal University, Beijing 100875, China
²National Institute of Biological Sciences, Zhongguancun Life Science Park, Beijing 102206, China
³Tsinghua-Peking Center for Life Sciences, Beijing 100084, China
⁴School of Life Sciences, Tsinghua University, Beijing 100084, China
⁵Graduate Program, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China
⁶College of Biological Sciences, China Agricultural University, Beijing 100193, China
⁷Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai 200032, China
⁸Michael Smith Laboratories, University of British Columbia, Vancouver, BC, Canada V6T 1Z4
Correspondence: Yijun, Qi,(qiyijun@biomed.tsinghua.edu.cn)

microRNAs (miRNAs) play important roles in the regulation of gene expression. In Arabidopsis, mature miRNAs are processed from primary miRNA transcripts (pri-miRNAs) by nuclear HYL1/SE/DCL1 complexes that form Dicing bodies (D-bodies). Here we report that an RNA-binding protein MOS2 binds to pri-miRNAs and is involved in efficient processing of pri-miRNAs. MOS2 does not interact with HYL1, SE, and DCL1 and is not localized in D-bodies. Interestingly, in the absence of MOS2, the recruitment of pri-miRNAs by HYL1 is greatly reduced and the localization of HYL1 in D-bodies is compromised. These data suggest that MOS2 promotes pri-miRNA processing through facilitating the recruitment of pri-miRNAs by the Dicing complexes.

Cell Research (2013) 23:645–657. doi:10.1038/cr.2013.23; published online 12 February 2013

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