Volume 3 Issue 2, July 1993: 187-193
ORIGINAL ARTICLES
Spatiotemporal distribution of 1P1 antigen expression in the plexiform layers of developing chick retina*
Houhua Wang1, Qiubao Song2 and Schloβhauer Burkhard3,4
1Shanghai Institute of Physiology, Germany
2Shanghai Institute of Cell Biology, Germany
3Max-Planck-Institute für Entwicklungsbiologic Tübingen, Germany
4Present address: Naturwissenschaftliches und Medizisches Institut an der Universit,it Tiibingen in Reutlingen, Germany
Correspondence: Houhua Wang
Changes in the distribution of 1Pl-antigen in the developing chick retina have been examined by indirect immunofluorescence staining technique using the novel monoclonal antibody ( MAb) 1P1. Expression of the 1P1 antigen was found to be regulated in radial as well as in tangential dimension of the retina, being preferentially or exclusively located in the inner and outer plexiform layers of the neural retina depending on the stages of development. With the onset of the formation of the inner plexiform layer 1P1 antigen becomes expressed in the retina. With progressing differentiation of the inner plexiform layer 1P1 immunofluorescence revealed 2 subbands at E9 and 6 subbands at E18. At postnatal stages (after P3) immunoreactivity was reduced in an inside-outside sequence leading to the complete absence of the 1P1 antigen in adulthood. 1P1 antigen expression in the outer plexiform layer was also subject to developmental regulation. The spatio-temporal pattern of 1P1 antigen expression was correlated with the time course of histological differentiation of chick retina, namely the synapse rich plexiform layers. Whether the 1P1 antigen was functionally involved in dendrite extension and synapse formation was discussed.
Cell Res 3: 187-193; doi:10.1038/cr.1993.20
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