Volume 3 Issue 1, January 1993: 21-26
ORIGINAL ARTICLES
The interaction of laminin and its membrane receptor on mouse macrophage membrane studied by STM and FRAP
Xinhua Wei1,3, Yong Zhao1, Xiaoming Dong1, Yaxian Su1, Zili Ma1,2, Changxin Zhu1,2 and Shijin Pang1,2
1Dept. of Cell Biology, Beijing Medical University, Beijing 100084, China.
2Beijing Vacuum Physics Laboratory, Academia Sinica, Beijing 100083, China.
3Present address: Research Center of Material Science, Science & Technology Section, Beijing Institute of Technology, Beijing 100081, China
Correspondence:
The variation of membrane surface and lateral diffusion of membrane protein was studied after the interaction of laminin with its membrane receptor in mouse macrophages. A pattern of membrane surface which showed smaller and bigger peaks was obtained by scanning tunneling microscope(STM), looking like the domains of lipid groups and proteins in the model of fluid mosaic biomembrane. Some even more higher and wider peaks projected out from the membrane surface in STM image after the interacting of laminin with membrane receptor were, probably, the complexes of laminin and membrane receptor. Furthermore, the decreased lateral diffusion coefficient value () was obtained by fluorescence recovery after photobleaching (FRAP) after the laminin was reacted with membrane receptor. This phenomenon provides an evidence that the complexes of laminin and its membrane receptor were located on the membrane of macrophages. So we could consider that the laminin is combined with membrane receptor leading to the variation in the properties of membrane surface.
Cell Res 3: 21-26; doi:10.1038/cr.1993.3
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