Volume 3 Issue 1, January 1993: 75-83
ORIGINAL ARTICLES
Antisense EGFR sequence reverses the growth properties of human liver carcinoma cell line BEL-7404 in vitro
Yonghua Xu, Wanli Jiang, Sufeng Peng and Yinghua Chen
Laboratory of Cellular and Molecular Oncology, Shanghai Institute of Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China
Correspondence:
A recombinant plasmid containing a full length human epidermal growth factor receptor (EGFR) cDNA sequence in antisense orientation was transferred into cells of a human liver carcinoma cell line BEL-7404. Compared with the control cell clone JX-0 transferred with the vector plasmid and the parent BEL-7404 cells, the antisense EGFR transferred cell clone JX-1 showed a decreased EGFR gene expression and reduced significantly the growth potential either in anchorage-dependent or anchorage-independent growth. Furthermore, JX-1 cells appeared to be distinctly dependent on serum concentration for monolayer growth. The results suggested that antisense EGFR could partly block the EGFR gene expression and reverse the malignant growth properties of human liver carcinoma cells in vitro.
Cell Res 3: 75-83; doi:10.1038/cr.1993.8
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