Volume 3 Issue 1, January 1993: 103-110
ORIGINAL ARTICLES
A DNA-binding protein factor in K562 nuclear extract interacts with positive control region (PCR) in the 5′-flanking sequence of human β-globin gene
Yulong Hu, Yadi Chen, Tong Sun and Ruolan Qian
Shanghai Institute of Cell Biology, Academia Sinica, Shanghai 200031, China.
Correspondence:
It has been known that there are at least three regulatory regions (NCR1, NCR2 and PCR) in the 5′-flanking sequence (from −610 bp to +1 bp) of human β-globin gene and that the function of PCR is unique to the human erythroleukemia (K562) cells. Here we have detected a DNA-binding protein factor (termed NFEa) in K562 cells, which can bind specifically to the PCR of human β-globin gene. The sequence of the binding site is 5′ ACTGATG3′ (between −222 bp and −216 bp). The NFEa is erythroid-specific and perhaps specific for K562 cells. It seemed that this factor differed from the erythroid-specific transcriptional factor (NFE-1) using competition assay. The presence of the NFEa further supported that the function of the cis-acting element PCR was specific for K562 cells, and helps us to understand the mechanism of the regulation of the expression of human β-globin gene in the human K562 cells.
Cell Res 3: 103-110; doi:10.1038/cr.1993.11
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