Volume 2 Issue 2, July 1992: 139-152
ORIGINAL ARTICLES
In Vitro transformation of LW13 rat liver epithelial cells
Can Shi1, Karl Letnansky2 and Zhen Yao1
1Shanghai Institute of Cell Biology, Academia Sinica, Shanghai 200031, China
2Institute of Applied and Experimental Oncology, University of Vienna, A 1090, Vienna, Austria
Correspondence:
A rat liver epithelial cell line designated LW13 was established using a sequential sedimentation method. The cell line retained many normal properties of liver epithelial cells and showed some structural and functional features resembling those of liver parenchymal cells. LW13 cells became malignant after the introduction of exogenous transforming EJ Ha ras gene. Tumors produced by inoculation of the transformed cells into baby rats contained areas of poorly differentiated hepatocellular carcinoma. In situ hybridization analysis confirmed the random rather than specific integration of exogenous ras gene into host chromosomes. Furthermore, an at least tenfold increase in the expression of the endogenous c myc gene was detected among transformed cell lines, suggesting the involvement of the c myc proto oncogene in the in vitro transformation of rat liver epithelial cells by EJ Ha ras oneogene.
Cell Res 2: 139-152; doi:10.1038/cr.1992.14
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