Volume 2 Issue 1, January 1992: 25-34
ORIGINAL ARTICLES
Construction of the physical map of yeast (Saccharomyces cerevisiae) chromosome V
Yiwen Zhu and Darren Kuang
Shanghai Institute of Cell Biology, Academia Sinica. Shanghai, China
Correspondence:
There are about 17 chromosomes in yeast Saccharomyces cerevisiae. A middle sized chromosome, chromosome V, was chosen in this work for studying and constructing the physical maps. Chromosome V from strain A364a was isolated by pulsed-field gradient gel electrophoresis (PFGE). Gel slices containing chromosome V DNA were digested with two rare cutting enzymes, NotI and SfiI, and three 6-Nt recognizing enzymes, SmaI, SstII and ApaI. Several strategies—partial or complete digestions, digestion with different sets of two enzymes, and hybridizabion with cloned genetically mapped probes (CAN1, URA3, CEN5, PRO3, CHO1, SUP19, RAD51, RAD3)—were used to align the restriction fragments. There are 9, 9, 15, 17, and 20 sites for NotI, SfiI, SmaI, SstII and ApaI respectively in the map of the A364a chromosome V. Its total length was calculated to be 620 Kb(Kilo-bases). The distributions of the cutting sites for these five enzymes through the whole chromosome are not uniform. A comparison between the physical map and the genetic map was also made.
Cell Res 2: 25-34; doi:10.1038/cr.1992.3
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