Volume 2 Issue 1, January 1992: 35-43
ORIGINAL ARTICLES
The development of the region between the preexisting and nascent membranes during the first cleavage of Rana amurensis eggs
Qirong Gao, Konghua Zhang, Longsheng Hong, Chengtang Xu and Guoyan Gu (formerly Kuoyen Ku)
Shanghai Institute of Cell Biology, Academa Sinica
Correspondence:
The region between the preexisting and nascent membranes of a cleaving Rana egg is a dense protrusion region of nearly 40 μm wide at 180° stage. Later, this region differentiate, into an upper part, a strip with long, branched and randomly distributed protrusions which are derived largely from the preexisting membrane, and a lower part, a band with microvilli. During 4-and 8-cell stages, the strip is almost vanished and microvilli in the band is shortened. The nascent membrane is smooth at the 180° stage, then a rough area appears below the smooth region and quickly expands. Wheat germ agglutinin which can bind to preexisting membrane interrupts the development of the region between the preexisting and nascent membranes. Detergent, Brij, having the property to increase the area of nascent membrane, does not interrupt the development of the region between the preexisting and nascent membranes.
Cell Res 2: 35-43; doi:10.1038/cr.1992.4
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