Volume 2 Issue 1, January 1992: 67-81
ORIGINAL ARTICLES
Fine analysis of the chromatin structure of yeast RNA polymerase II transcription terminators
Gengxi Hu, Yunhua Yu and Darren Kuang
Shanghai Institute of Cell Biclogy, Academia Sinica. 320 Yue-Y ang Road, Shanghai 200031, China
Correspondence:
In order to study the functional structure of the transcription terminators and the mechanism of termination, a survey of the chromatin structure, including the location of DNase I hypersensitive sites and the nucleosome arrangement, of yeast ADHI and FLP terminators was made. The results show that there is no relationship between the function of the terminators and the existence of DNase I hypersensitive sites. However, it is found that there is always a nucleosome at the immediate upstream of the transcriptional termination sites. As a control, the chromatin structures of the pBR322 DNA fragments on the yeast shutter vectors are also investigated at the same time. The random nucleosome arrangement on lhe bacterial DNA in yeast agrees with the published reports. A new hypothesis, about the mechanism of transcriptional termination is put forward and the reason of different nucleosome arrangement on the DNAs which are originally from different species in yeast is discussed.
Cell Res 2: 67-81; doi:10.1038/cr.1992.7
FULL TEXT | PDF
Browse 2143
