Volume 1 Issue 2, December 1990: 131-139
ORIGINAL ARTICLES
Construction and packaging of pseudotype retrovirus containing human N-ras cDNA antisense sequence and its biological effects on human hepatoma cells
Libin Jia, Xiang Wang, Xiulan Xu, Jiqing Ma, Jin-Ying Yang, Chaoting Liu and Jianren Gu
National Research Center for Orcogenes and Related Genes, Shanghai Cancer Institute, China
Correspondence:
N-ras is one of the transforming genes in human hepatic cancer cells. It has been found that N-ras was overexpressed at the mRNA and probein level in hepatoma cells. In order to explore the biological roles of N-ras in human hepatic carcinogenesis and the potential application in control of cancer cell growth, a pseudotype retrovirus containing antisense sequence of human N-ras was constructed and packaged. A recombinant retrovirus vector containing antisense or sense sequences of N-ras cDNA was constructed by pZIP-NeoSV (X) 1. The pseudotype virus was packaged and rescued by transfection and infection in PA317 and ψ2 helper cells. It has been demonstrated that the pseudotype retrovirus containing antisense N-ras sequence did inhibit the growth of human PLC/PRF/5 hepatoma cells accompanied with inhibition of p21 expression, while the retrovirus containing sense sequence had none. The pseudotype virus had no effect on human diploid fibroblasts.
Cell Res 1: 131-139; doi:10.1038/cr.1990.13
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