Volume 1 Issue 2, December 1990: 163-172
ORIGINAL ARTICLES
Antiidiotypic antibody related to the 84 kD human sperm membrane protein
Jun Yu1, Linfang Wang2, Shiying Miao2 and Yuanchang Yan1,1
1Shanghai Institute of Cell Bioloogy, Academia Sinica, Shanghai 200031, China
2Institute of Basic Medical Sciences, CAMS, Beijing 100730, China
Correspondence:
Wistar rats were inoculated with purified YWK-I antibody. The anti-idiotypic antibodies were isolated from rat sera by successive passage over affinity chromatography columns of YWK-I mAb and normal mouse Igs. Specificity of anti-Id antibody was established by ELISA. The 84 kD protein inhibited the binding of anti-Id to YWK-I mAb, but failed to repress antibody against normal mouse Ig binding to YWK-I mAb. In competitive inhibition assay, 84kD protein had shown the ability to compete with anti-Id binding to YWK-I mkb in a dose-dependent manner. Crude sperm extract showed a lower competitive ability. No effect was found with the irrelevant 36 kD sperm protein. The antisera from the Balb/c mice immunized with Aid contained Ab3 that reacted with 84 kD sperm protein. The binding of anti-Id to YWK-I mAb was inhibited by Ab3 in a dose-dependent fashion and Ab3 was shown to be able to induce human sperm agglutination. These results indicate that anti-Id which may mimic an epitope of the 84 kD protein could be exploited as an antigen to raise antibodies against sperm protein.
Cell Res 1: 163-172; doi:10.1038/cr.1990.16
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