Volume 1 Issue 2, December 1990: 191-196
ORIGINAL ARTICLES
Pollen-embryogenesis and chromosomal variability in anther culture of Brassica hirta Moench (Sinapis alba L)
Bajaj YPS1 and D Mohaptra2
1A-137 New Friends Colouy, New Delhi 110065, India
2NARP, R.R.S, Ranital, Balasore Orissa 756111, India
Correspondence:
The anther cultures of Brassica hirta underwent pollen-embryogenesis and callusing, which showed a wide range of chromosome numbers varying from 9 (n=12) to a highly polyploid. For embryogenesis, pretreatment of floral buds in 0.4M sucrose solution for 72 hrs at 4°C was superior to freshly cultured anthers. Culture temperature of 30°C for 14 days before maintenance of cultures at 25°C was significantly beneficial for embryo yield in comparison to cultures conlinuously incubated at 25°C. Dark treatment during culture was more effective for pollen-embryo yield.
Cell Res 1: 191-196; doi:10.1038/cr.1990.19
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