Volume 1 Issue 1, March 1990: 17-21
ORIGINAL ARTICLES
Plant regeneration from cultured protoplasts of a glutinous rice
Guangyuan Wang, Chenau Hsia (Zhenao Xia), Jinfen Chi and Xiaoyan Gao
Shanghai Institute of Plant Physiology, Academia Sinica
Correspondence:
Young embryos of rice (Oryza sativa L. subsp, japonica var. Guo-xiang No. I) were cultured on MS agar medium(2,4-D 2 mg/l). Calli were formed and subcultured on N6 agar medium (2,4-D 2 mg/l). After selection, the small, grainy and pale yellowish cell clusters with dense cytoplasm were used in protoplast preparation. Isolated protoplasts were cultured in N6 medium(2,4-D 1 mg/l, 6-BA 0.2 mg/l)1* with agarose block culture method. The protoplasts grew, divided and formed calli. After inducing differentiation, the regenerated mature plants were obtained.
Cell Res 1: 17-21; doi:10.1038/cr.1990.3
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