Volume 1 Issue 1, March 1990: 23-33
ORIGINAL ARTICLES
In vitro Induction of primary antibody responses to particulate and soluble protein antigens in T cell-replaced murine spleen cell cultures
Kun Lu
Shanghai Institute of Cell Biology, Academia Sinica, Shanghai, 200031, China
Correspondence:
Specific antibody responses could be induced in serum, free condition. Specific anti-SRBC or anti-SRBC ghost antibody were induced from anti-Thy treated (T-depleted) murine spleen cells in serum-free culture in the presence of Con A conditioned medium. This induction system may facilitate the study of lymphokine functions on antigen triggered B cells.
In T cell-replaced cultures, the antibody responses of B cells could be successfully induced when soluble SRBC membrane proteins were used as antigens. It thus indicates that antigen together with lymphokines are sufficient to drive B cells to become antibody secreting cells in the absence of T cells. The T cell-replaced system provides a more stable way for in vitro immunization and may be applied to monoclonal antibody production when in vivo immunization is difficult to be carried out.
Cell Res 1: 23-33; doi:10.1038/cr.1990.4
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