Volume 1 Issue 1, March 1990: 35-51
ORIGINAL ARTICLES
Effects of feeder layer and BRL conditioned medium on mouse embryonic stem cells
Hsiaochien Tsung1 and Christine L. Mummery2
1Shanghai Institute of Cell Biology, Academia Sinica
2Netherlands Institute for Development Biology, Utrecht, Netherlands
Correspondence:
In vitro growth and maintenance of embryonic stem (ES) cell lines derived from ICM cells of various blastocysts of 129 strain mice, the sustenance of their pluripotency and normal karyotype depend on the feeder layer of mouse embryonic fibroblasts (MEF). Compared with the feeder layer of MEF cells, medium conditioned by Buffalo rat liver cells (BRL-CM) is able to maintain pluripotency and karyotypic normality of ES cells only in short term cell propagation. Besides, ES cells grown in BRL-CM are also capable of aggregation with 8-cell embryos of Swiss strain and develop into germ line chimaeras. Modification to the method of aggregating ES cells with early embryos by making a hole in agar layer on the top of MEF feeder cells was shown to be more convenient and efficient than the conventional microdrop method.
Cell Res 1: 35-51; doi:10.1038/cr.1990.5
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