Volume 1 Issue 1, March 1990: 105-118
ORIGINAL ARTICLES
The effects of DNA intercalators on chromatin of chicken red blood cells --- differential extraction on nonhistone proteins
Fan Wang and Jingde Zhu
Shanghai Institute of Cell Biology; Shanghai Open Labortory of Life Science, Academia Sinica, 320 Yue-Yang Road, Shanghai 200031, China
Correspondence:
Taking advantage of the effects on DNA secondary structure of two DNA-intercalators, ethidium bromide and chloroquine, we used each of them to treat nuclei from both mature erythrocytes and reticulocytes of chicken, as an alternative approach to study the relationships between DNA secondary structure, nuclear proteins and chromatin structure. We presented results of differential extraction of nuclear proteins from nuclei with DNA-intercalators, as well as preliminary characterization of these proteins. A 45kd protein is the major component in fractions extracted by both intercalators from nuclei from either mature erythrocytes or reticulocytes and seems to be a DNA-binding protein. Furthermore, from current concepts of functional aspects of DNA conformation and structural heterogeneity in chromatin and nuclear proteins, we have discussed both the significance of our resuits as well as technical aspects of this approach.
Cell Res 1: 105-118; doi:10.1038/cr.1990.11
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