Volume 23, No 6, Jun 2013

ISSN: 1001-0602 
EISSN: 1748-7838 2018 
impact factor 17.848* 
(Clarivate Analytics, 2019)

Volume 23 Issue 6, June 2013: 733-734

RESEARCH HIGHLIGHTS

RNA-guided genome editing à la carte

Philippe Horvath¹ and Rodolphe Barrangou^2,3

¹DuPont Nutrition and Health, Dangé-Saint-Romain, 86220, France
²DuPont Nutrition and Health, Madison, WI 53716, USA
³New address: Department of Food, Bioprocessing and Nutrition Sciences, North Carolina State University, Raleigh, NC 27695, USA Correspondence: Philippe Horvath, Tel: +33-549-191-209; Fax: +33-549-864-839(philippe.horvath@dupont.com)

Two recent papers in Science illustrate how the prokaryotic CRISPR-Cas immune system machinery, which typically targets invasive genetic elements such as viruses and plasmids, can be converted into a sophisticated molecular tool for next-generation human genome editing. The versatile Cas9 RNA-guided endonuclease can be readily reprogrammed using customizable small RNAs for sequence-specific single- or double-stranded DNA cleavage.

10.1038/cr.2013.39

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