Volume 23, No 10, Oct 2013

ISSN: 1001-0602 
EISSN: 1748-7838 2018 
impact factor 17.848* 
(Clarivate Analytics, 2019)

Volume 23 Issue 10, October 2013: 1187-1200

ORIGINAL ARTICLES

Generation of haploid embryonic stem cells from Macaca fascicularis monkey parthenotes

Hui Yang^1,*, Zhen Liu^2,*, Yu Ma^3,*, Cuiqing Zhong¹, Qi Yin¹, Chikai Zhou^1,4, Linyu Shi¹, Yijun Cai², Hanzhi Zhao³, Hui Wang⁵, Fan Tang³, Yan Wang², Chenchen Zhang², Xin-yuan Liu⁴, Dongmei Lai⁵, Ying Jin³, Qiang Sun² and Jinsong Li¹

¹Group of Epigenetic Reprogramming, State Key Laboratory of Cell Biology, Shanghai Key Laboratory of Molecular Andrology, Institute of Biochemistry and Cell Biology, Shanghai Jiao Tong University School of Medicine, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China
²Institute of Neuroscience, Shanghai Jiao Tong University School of Medicine, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China
³Key Laboratory of Stem Cell Biology, Institute of Health Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China
⁴College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, Zhejiang 310018, China
⁵The International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200030, China Correspondence: Jinsong Li, E-mail: jsli@sibcb.ac.cn; Qiang Sun, E-mail: qsun@ion.ac.cn; Ying Jin(yjin@sibs.ac.cn)

Recent success in the derivation of haploid embryonic stem cells (haESCs) from mouse via parthenogenesis and androgenesis has enabled genetic screening in mammalian cells and generation of gene-modified animals. However, whether haESCs can be derived from primates remains unknown. Here, we report the derivation of haESCs from parthenogenetic blastocysts of Macaca fascicularis monkeys. These cells, termed as PG-haESCs, are pluripotent and can differentiate to cells of three embryonic germ layers in vitro or in vivo. Interestingly, the haploidy of one monkey PG-haESC line (MPH1) is more stable compared with that of the other one (MPH2), as shown by the existence of haploid cells for more than 140 days without fluorescence-activated cell sorting (FACS) enrichment of haploid cells. Importantly, transgenic monkey PG-haESC lines can be generated by lentivirus- and piggyBac transposon-mediated gene transfer. Moreover, genetic screening is feasible in monkey PG-haESCs. Our results demonstrate that PG-haESCs can be generated from monkeys, providing an ideal tool for genetic analyses in primates.

10.1038/cr.2013.93

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