Volume 23, No 11, Nov 2013

ISSN: 1001-0602 
EISSN: 1748-7838 2018 
impact factor 17.848* 
(Clarivate Analytics, 2019)

Volume 23 Issue 11, November 2013: 1322-1325

LETTERS TO THE EDITOR

Heritable gene-targeting with gRNA/Cas9 in rats

Xinli Hu¹, Nannan Chang¹, Xuelian Wang¹, Fengyun Zhou¹, Xiaohai Zhou¹, Xiaojun Zhu¹ and Jing-Wei Xiong¹

¹Beijing Key Laboratory of Cardiometabolic Molecular Medicine, Institute of Molecular Medicine, Peking University, Beijing, China Correspondence: Jing-Wei Xiong, Tel: 8610-62766239 E-mail: jingwei_xiong@pku.edu.cn; Xinli Hu,(huxxx025@pku.edu.cn)

The rat is a preferred animal model in many research applications, especially in physiological, behavioral and translational studies1. Although the rat embryonic stem (ES) cells were successfully isolated2,3, the rat ES cell-based gene-targeting approach has not been widely adopted due to the technical difficulties in manipulating these cells. The first knockout rat was generated via microinjection of zinc finger nuclease (ZFN) into the embryos4.

10.1038/cr.2013.141

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