Volume 24, No 1, Jan 2014

ISSN: 1001-0602 
EISSN: 1748-7838 2018 
impact factor 17.848* 
(Clarivate Analytics, 2019)

Volume 24 Issue 1, January 2014: 126-129

LETTERS TO THE EDITOR

Small molecules enable OCT4-mediated direct reprogramming into expandable human neural stem cells

Saiyong Zhu¹, Rajesh Ambasudhan², Woong Sun³, Hyun Jung Kim³, Maria Talantova², Xiaojing Wang¹, Mingliang Zhang¹, Yu Zhang¹, Timothy Laurent¹, James Parker², Han-Seop Kim⁴, Jeffrey D Zaremba², Sofiyan Saleem², Sara Sanz-Blasco², Eliezer Masliah⁵, Scott R McKercher², Yee Sook Cho⁴, Stuart A Lipton^2,5, Janghwan Kim^1,4 and Sheng Ding¹

¹Gladstone Institute of Cardiovascular Disease, Department of Pharmaceutical Chemistry, University of California, San Francisco, 1650 Owens Street, San Francisco, CA 94158, USA
²Del E Webb Center for Neuroscience, Aging, and Stem Cell Research, Sanford-Burnham Medical Research Institute, 10901 North Torrey Pines Road, La Jolla, CA 92037, USA
³Department of Anatomy, Korea University College of Medicine, Seoul 136-705, Korea
⁴Stem Cell Research Center, Korea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806, Korea
⁵Department of Neurosciences, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA Correspondence: Sheng Ding, Tel: 415-734-2717; Fax: 415-355-0141 E-mail: sheng.ding@gladstone.ucsf.edu; Janghwan Kim, Tel: +82-42-860-4478; Fax: +82-42-879-8495(janghwan.kim@kribb.re.kr)

We previously developed a novel paradigm of cell activation and signaling-directed (CASD) lineage conversion for direct reprogramming of fibroblasts into cardiac, neural and endothelial precursor cells. This method is based on the transient overexpression of iPSC factors (cell activation, CA) in conjunction with lineage-specific soluble signals (signaling directed, SD). Such a strategy was used to generate human induced neural stem cells (hiNSCs) with 4 to 6 pluripotency factors, including OCT4, SOX2, KLF4 and microRNAs, from cells in human urine1 and fibroblasts2. Using a different strategy, SOX2 was found to induce the generation of hiNSCs, although only from fetal fibroblasts with low efficiency and by a tedious process3.

10.1038/cr.2013.156

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