Volume 24, No 2, Feb 2014

ISSN: 1001-0602 
EISSN: 1748-7838 2018 
impact factor 17.848* 
(Clarivate Analytics, 2019)

Volume 24 Issue 2, February 2014: 247-250

LETTERS TO THE EDITOR

Oligonucleotide-based targeted gene editing in C. elegans via the CRISPR/Cas9 system

Pei Zhao^1,*, Zhe Zhang^1,*, Hongmei Ke¹, Yiren Yue¹ and Ding Xue^1,2

¹School of Life Sciences, Tsinghua University, Beijing 100084, China
²Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Colorado 80309, USA Correspondence: Ding Xue,(ding.xue@colorado.edu)

Technologies to achieve specific and precise genome editing, such as knock-in and knock-out, are critical for deciphering the functions of a gene and for understanding fundamental biological processes. Compared with the zinc finger nucleases (ZFN) and transcription activator-like effector nucleases (TALEN), which have been used for genome editing1, the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated (Cas) system has emerged as a new powerful tool for genome modifications. It has recently been adopted for genome editing in human cell lines2,3,4, mouse5, zebrafish6, C. elegans7,8,9,10,11,12, and plants13.

10.1038/cr.2014.9

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