Volume 24, No 3, Mar 2014

ISSN: 1001-0602 
EISSN: 1748-7838 2018 
impact factor 17.848* 
(Clarivate Analytics, 2019)

Volume 24 Issue 3, March 2014: 372-375   |  Open Access

LETTERS TO THE EDITOR

One-step generation of knockout pigs by zygote injection of CRISPR/Cas system

Tang Hai¹, Fei Teng^1,2, Runfa Guo¹, Wei Li¹ and Qi Zhou¹

¹State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
²University of Chinese Academy of Sciences, Beijing 100049, China Correspondence: Qi Zhou, Fax: 86-10-64807299(qzhou@ioz.ac.cn)

The pig is an important livestock for food supply and an ideal model for various human diseases. Efficient and precise genetic engineering in pigs holds great promise in agriculture and biomedicine1. Using currently available approach, generating specific gene modifications in pigs requires two steps. First, site-specific nucleases such as zinc finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs) are used to generate targeted mutations in pig somatic cells. Then the engineered somatic nucleus is used to generate cloned animals using somatic cell nuclear transfer (SCNT) technology2,3. The complex design and generation of ZFNs and TALENs, as well as the technical challenges of SCNT, greatly limit the application of this method.

10.1038/cr.2014.11

FULL TEXT | PDF

Browse 2274