Volume 24, No 7, Jul 2014

ISSN: 1001-0602 
EISSN: 1748-7838 2018 
impact factor 17.848* 
(Clarivate Analytics, 2019)

Volume 24 Issue 7, July 2014: 886-889

LETTERS TO THE EDITOR

Heritable/conditional genome editing in C. elegans using a CRISPR-Cas9 feeding system

¹The Education Ministry Key Laboratory of Cell Proliferation and Differentiation and the State Key Laboratory of Bio-membrane and Membrane Bio-engineering, School of Life Sciences, Peking University, Beijing, China ²Institute of Molecular Medicine, Peking University, Beijing, China

Correspondence: Dong Liu,(E-mail: doliu@pku.edu.cn)

Type II clustered, regularly interspaced, short palindromic repeat (CRISPR)-associated (Cas) system is a novel genome-editing tool for targeted mutagenesis in cultured cells and whole organisms1,2,3. Recently, the CRISPR-Cas9 system has been applied in C. elegans using various delivery approaches including the transgene method and the direct injection of Cas9 mRNA/single guide RNA (sgRNA) or Cas9-sgRNA ribonucleoproteins4,5,6. In the present study, we have developed a CRISPR-Cas9-based genome-editing tool for C. elegans by delivering gene-specific guide RNA (gRNA) through bacterial feeding to achieve gene disruptions in a time- and labor-saving manner.

10.1038/cr.2014.73

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