Volume 24, No 12, Dec 2014
ISSN: 1001-0602
EISSN: 1748-7838 2018
impact factor 17.848*
(Clarivate Analytics, 2019)
Volume 24 Issue 12, December 2014: 1445-1465 | Open Access
ORIGINAL ARTICLES
SUVR2 is involved in transcriptional gene silencing by associating with SNF2-related chromatin-remodeling proteins in Arabidopsis
Yong-Feng Han1,*, Kun Dou1,*, Ze-Yang Ma1, Su-Wei Zhang1, Huan-Wei Huang1, Lin Li1, Tao Cai1, She Chen1, Jian-Kang Zhu2,3 and Xin-Jian He1
1National Institute of Biological Sciences, Beijing 102206, China
2Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907, USA
3Shanghai Center for Plant Stress Biology and Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China
Correspondence: Xin-Jian He, Tel: +86-10-8070-7712; Fax: +86-10-8070-7715(hexinjian@nibs.ac.cn)
The SU(VAR)3-9-like histone methyltransferases usually catalyze repressive histone H3K9 methylation and are involved in transcriptional gene silencing in eukaryotic organisms. We identified a putative SU(VAR)3-9-like histone methyltransferase SUVR2 by a forward genetic screen and demonstrated that it is involved in transcriptional gene silencing at genomic loci targeted by RNA-directed DNA methylation (RdDM). We found that SUVR2 has no histone methyltransferase activity and the conserved catalytic sites of SUVR2 are dispensable for the function of SUVR2 in transcriptional silencing. SUVR2 forms a complex with its close homolog SUVR1 and associate with three previously uncharacterized SNF2-related chromatin-remodeling proteins CHR19, CHR27, and CHR28. SUVR2 was previously thought to be a component in the RdDM pathway. We demonstrated that SUVR2 contributes to transcriptional gene silencing not only at a subset of RdDM target loci but also at many RdDM-independent target loci. Our study suggests that the involvement of SUVR2 in transcriptional gene silencing is related to nucleosome positioning mediated by its associated chromatin-remodeling proteins.
10.1038/cr.2014.156
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