Volume 25, No 2, Feb 2015
ISSN: 1001-0602
EISSN: 1748-7838 2018
impact factor 17.848*
(Clarivate Analytics, 2019)
Volume 25 Issue 2, February 2015: 254-257 | Open Access
LETTERS TO THE EDITOR
Large-field high-resolution two-photon digital scanned light-sheet microscopy
Weijian Zong1,2, Jia Zhao1, Xuanyang Chen1, Yuan Lin1, Huixia Ren1, Yunfeng Zhang4, Ming Fan2, Zhuan Zhou1, Heping Cheng1, Yujie Sun3 and Liangyi Chen1
1The State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Molecular Medicine, Beijing Key Laboratory of Cardiometabolic Molecular Medicine, Institute of Molecular Medicine, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871, China
2China Department of Cognitive Sciences, Institute of Basic Medical Sciences, Beijing 100850, China
3Biodynamic Optical Imaging Center, Peking University, Beijing 100871, China
4School of Electronics Engineering and Computer Science, Peking University, Beijing 100871, China
Correspondence: Liangyi Chen, Tel: +86-10-62764959 E-mail: lychen@pku.edu.cn; Yujie Sun,(sun_yujie@pku.edu.cn)
Recent advent of light-sheet fluorescent microscopy (LSFM) has revolutionized three-dimensional biological imaging with high temporal resolution and minimal photodamage, enabling long-term fluorescence imaging of tissues and small organisms1,2. By combining two-photon fluorescence excitation with LSFM, Truong et al.3 have created a two-photon digital scanned light-sheet microscope (2P-DSLM), allowing for deep-tissue imaging of highly scattering Drosophila embryos and fast beating hearts of zebrafish. Similar to classical LSFM configurations, a 2P-DSLM uses a low numerical aperture (NA < 0.1) to achieve a long and homogenous illumination. This leads to a thick light sheet and thus reduces axial resolution and image contrast. On the other hand, Betzig and colleagues have used a Bessel beam to generate thin single-photon light sheet that yields superb axial resolution4. However, the field of view and the penetration depth are limited in such system.
10.1038/cr.2014.124
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